20th International AIDS Conference - Melbourne, Australia

Abstract

MOPE119 - Poster Exhibition


Putative case of HIV-1 transmission related to cuticle pliers

V. Pimentel1,2, L. Coelho2, C.J. Villabona1, C. Hársi1, H. Onias3, L. Brígido2, E. Matsuda3

1University of São Paulo, Microbiology, São Paulo, Brazil, 2Adolfo Lutz Research Institute, São Paulo, Brazil, 3Santo Andre Aids Program, Santo Andre, Brazil

Background: Body fluids can contaminate different objects with infectious organisms and lead to fomite transmission of diseases. Negligence when using beauty utensils (such as needles for tattooing and razors for shaving) poses a risk for virus transmission. Here, we document an event of HIV-1 transmission potentially associated the sharing of cuticle plier.
Methods: A female diagnosed with HIV-1 infection at blood donotion reported no sexual intercourse or intimate contact as well as other potential sources of known HIV transmission. Nonetheless, she informed non-sexual contact with a HIV-1 infected female cousin. Both the relative (putative index case, PIC) and the newly diagnosed patient, had DNA from cells and RNA from plasma extracted at different time points. The genomic material was used to amplify and sequence the env-gp120 region. These sequences were evaluated with available Brazilian time-stamped (1989-2013) reference sequences of HIV-1 subtype B retrieved from GenBank (6800-7500 HXB-2 positions) and other available sequences from patients'' city. This dataset was used in a phylogenetic analysis following a Bayesian approach and using a General Time Reversible nucleotide substitution model and a relaxed molecular clock.
Results: The phylogenetic analysis of the env-gp120 region revealed that both patients had a common ancestor. The group had a posterior probability of 1.0 and the likely transmission event was dated to 10.81 years ago (HPDs 4 -18). New interviews with the patients and relatives were performed to rule out adoption, transfusion, IDU or any other known mode of HIV transmission, with confirmation of sharing cuticle pliers around the likely estimated transmission period. Index case, now virally suppressed, had a viral load of 3.6 log at that time.
Conclusions: Both patients'' strains are phylogenetically related with a period of transmission (around 2003) that is consistent with epidemiological and virological findings. All together, results suggest the share of manicure paraphernalia as a possible route of HIV-1 transmission.

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